Montagem de um pseudo-hantavírus quimera, contendo a nucleoproteína do vírus Araraquara e as glicoproteínas do vírus Andes, em sistema baculovírus / Assembly of a chimeric hantavirus-like particle, containing the Araraquara nucleoprotein and the Andes glycoproteins, expressed in baculovirus system

AUTOR(ES)
DATA DE PUBLICAÇÃO

2010

RESUMO

Hantaviruses, members of the Bunyaviridae family, are the infectious agents responsible for Hemorrhagic Fever with Renal Syndrome and the Hantavirus Cardiopulmonary Syndrome. The viral genome is composed by three segments of single-stranded negative-sense RNA, designated as S, M and L, which encode, respectively, the nucleoprotein, the G1 and G2 glycoproteins, and the RNA-dependent RNA polymerase. In order to study the assembly of a chimeric hantavirus-like particle, the Araraquara nucleoprotein and the Andes glycoproteins were expressed in a baculovirus system. Confocal microscopy showed the colocalization of G1 and G2 proteins with the N protein. Immunoprecipitation assay and sucrose density gradient showed the interaction among N, G1 and G2 proteins. The transmission electron microscopy showed the hantavirus-like particle with the same morphology of the virion. The chimeric hantavirus-like particle produced in this study could be used, in the future, in immunological, structural and morphological studies.

ASSUNTO(S)

nucleoproteína virus protein expression nucleoprotein proteínas expressão de proteínas sistema baculovírus baculovirus system protein vírus glycoprotein glicoproteínas

ACESSO AO ARTIGO

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