Monovalent antibodies against MM-creatine kinase remove the M line from myofibrils.
AUTOR(ES)
Wallimann, T
RESUMO
Column-purified antibodies against creatine kinase (EC 2.7.3.2) from chicken skeletal muscle (the homodimeric isoenzyme designated MM-CK) bind specifically to the M lines of chicken pectoral muscle myofibrils. Incubation of myofibrils with monovalent Fab' fragments of these antibodies solubilizes most of the myofibril-bound creatine kinase, concomitantly removing most of the electron-dense material from the M lines. This strongly indicates that MM-CK is an integral part of the M-line structure and is consistent with the suggestion that MM-CK molecules form the M bridges that are responsible for the principle M-line substriations.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=336100Documentos Relacionados
- Mechanical characterization of skeletal muscle myofibrils.
- Passive and active tension in single cardiac myofibrils.
- Rotational dynamics of actin-bound intermediates of the myosin adenosine triphosphatase cycle in myofibrils.
- Calcium alone does not fully activate the thin filament for S1 binding to rigor myofibrils.
- Molecular cloning of a DNA sequence complementary to creatine kinase M mRNA from chickens.