Molecular Studies on the Role of a Root Surface Agglutinin in Adherence and Colonization by Pseudomonas putida†

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RESUMO

Pseudomonas putida aggressively colonizes root surfaces and is agglutinated by a root surface glycoprotein. Mutants of P. putida derived chemically or by Tn5 insertion demonstrated enhanced or decreased agglutinability. Two nonagglutinable Tn5 mutants (Agg−) and two mutants with enhanced agglutinability (Aggs) possessed Tn5 in unique restriction sites. Agg− mutants colonized root surfaces of seedlings grown from inoculated seeds, but at levels lower than those observed with the Agg+ parent. In short-term binding studies, Agg− cells adhered at levels that were 20- to 30-fold less than those for Agg+ parental cells. These data suggest that the agglutination interaction plays a role in the attachment of P. putida to root surfaces.

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