Molecular Insights into the Genetic Diversity of Garcinia cambogia Germplasm Accessions
AUTOR(ES)
Tharachand, C, Immanuel Selvaraj, C, Abraham, Z
FONTE
Braz. arch. biol. technol.
DATA DE PUBLICAÇÃO
2015-10
RESUMO
ABSTRACTIn this work, the genetic relationship among twelveGarcinia cambogia (Gaertn.) Desr. accessions were evaluated using Random Amplified Polymorphic DNA markers. The samples were part of the germplasm collected and maintained at NBPGR Regional station, Thrissur, India. Out of thirty RAPD primers used for screening, seven primers produced a total of 128 polymorphic markers in twelve accessions. The Polymorphic Information Content (PIC) ranged from 0.28 (OPA18) to 0.37 (OPA9) and Marker Index (MI) ranged between 3.61 (OPA12) and 5.93 (OPA3) among the primers used. Jaccard's coefficient of genetic similarity ranged between 0.07 and 0.64. The dendrogram constructed based on the similarity matrix generated from the molecular and morphological data showed the genetic relationship among the sampled accessions. Mantel matrix test showed a positive correlation (r = 0.49) between the cluster analysis of RAPD data and morphological data. The clustering pattern in the molecular dendrogram and Principle Coordinate Analysis (PCoA) showed that the genotypes were diverse, which was in congruence with the similarity index values and morphological dendrogram. High frequency of similarity values in the range of 0.11 to 0.17 suggested the existence of high genetic diversity among the accessions. The high level of genetic diversity among the studied accessions ofG.cambogia was also supported by the large variation in the morphological characters observed in the flowers, leaves, fruits and seeds of these sampled accessions. This is the first report for the molecular based genetic diversity studies for these accessions.
Documentos Relacionados
- Joint analysis of phenotypic and molecular diversity provides new insights on the genetic variability of the brazilian physic nut germplasm bank
- Molecular diversity in a germplasm collection of avocado accessions from the tropical and subtropical regions of China
- Chemical diversity of accessions of the in vivo germplasm bank of Varronia curassavica (Jacq.)
- Genetic diversity in Brazilian soybean germplasm
- Comparative evaluation of anthelmintic and antibacterial activities in leaves and fruits of Garcinia cambogia (Gaertn.) desr. and Garcinia indica (Dupetit-Thouars) choisy