Molecular Identification of Dialister pneumosintes in Subgingival Plaque of Humans
AUTOR(ES)
Doan, N.
FONTE
American Society for Microbiology
RESUMO
Dialister pneumosintes is a nonfermentative, anaerobic, gram-negative rod that grows with small, circular, transparent, shiny, smooth colonies on blood agar. Even though D. pneumosintes has been recovered from deep periodontal pockets, little is known about the relationship between the organism and destructive periodontal disease. This study describes a rapid PCR method to identify D. pneumosintes in periodontal samples. The PCR identification method detected as little as 10 pg of D. pneumosintes DNA or about 1 to 10 cells without nonspecific amplification of various periodontopathic bacteria. Twelve of 22 subgingival samples from adult periodontitis lesions yielded D. pneumosintes either by culture or by PCR identification. In culture-positive samples, D. pneumosintes averaged 3.9% (0.001 to 10.8%) of total isolates. Studies are needed to delineate virulence factors of D. pneumosintes pertinent to periodontal disease.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=87182Documentos Relacionados
- Dialister pneumosintes Associated with Human Brain Abscesses
- Identification of Actinobacillus actinomycetemcomitans in subgingival plaque by PCR.
- Identification of Bacteroides forsythus in subgingival plaque from patients with advanced periodontitis.
- Bacterial Diversity in Human Subgingival Plaque
- Hemagglutinating activity of lipopolysaccharides from subgingival plaque bacteria.
