Molecular diagnosis of strongyloidiasis in tropical areas: a comparison of conventional and real-time polymerase chain reaction with parasitological methods
AUTOR(ES)
Paula, Fabiana Martins de, Malta, Fernanda de Mello, Marques, Priscilla Duarte, Sitta, Renata Barnabé, Pinho, João Renato Rebello, Gryschek, Ronaldo César Borges, Chieffi, Pedro Paulo
FONTE
Mem. Inst. Oswaldo Cruz
DATA DE PUBLICAÇÃO
06/03/2015
RESUMO
This study aimed to evaluate the use of conventional polymerase chain reaction (cPCR) and real-time quantitative PCR (qPCR) in the diagnosis of human strongyloidiasis from stool samples in tropical areas. Stool samples were collected from individuals and were determined to be positive for Strongyloides stercoralis (group I), negative for S. stercoralis (group II) and positive for other enteroparasite species (group III). DNA specific to S. stercoralis was found in 76.7% of group I samples by cPCR and in 90% of group I samples by qPCR. The results show that molecular methods can be used as alternative tools for detecting S. stercoralis in human stool samples in tropical areas.
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