Mixed Ternary Mononuclear Copper(II) Complexes Based on Valproic Acid with 1,10-Phenanthroline and 2,2'-Bipyridine Ligands: DNA Interaction and Cytotoxicity in V79 Cells
AUTOR(ES)
Pich, Claus T.
FONTE
J. Braz. Chem. Soc.
DATA DE PUBLICAÇÃO
2019-03
RESUMO
This paper reports the DNA interaction and cytotoxicity of binary and ternary copper(II) complexes of valproic acid with phenanthroline or bipyridine and the complementary characterization of Cu(Valp)2Bipy by crystallography. Circular dichroism, plasmid and oligonucleotide assays have shown that the complexes interact with DNA in different manners and are able to cleave plasmid DNA and oligonucleotides at concentrations from 10 to 1000 mol L-1. The plasmid DNA cleavage is enhanced 650, 375 and 285 times by photo activation with UVB light for the complexes Cu(Valp)2Phen, Cu(Valp)2Bipy and Cu2(Valp)4, respectively. Cytotoxicity assays demonstrated that V79 cells were more sensitive to CuII derivatives than sodium valproate as evaluated by 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) and clonogenic assays, where the cytotoxic profile of the compounds was: Cu(Valp)2Phen higher than Cu(Valp)2Bipy, higher than Cu2(Valp)4. Therefore, we report two copper complexes, which interact with DNA and promote its cleavage, leading to an enhanced cytotoxicity when compared to valproic acid.
Documentos Relacionados
- The influence of reducing agent and 1,10-phenanthroline concentration on DNA cleavage by phenanthroline + copper.
- Effects of Polycyclic Aromatic Hydrocarbon Pendant-Armed Ligands on the Catecholase Activity of Dinuclear Copper(II) Complexes
- Two different modes for copper(II) ion coordination to quinine-type ligands
- STRUCTURE AND OPTICAL ACTIVITY IN METAL COMPLEXES, VI. INTERACTION OF HISTIDINE WITH COPPER(II) IN SOLUTION*
- Copper(II) Nitroaromatic Schiff Base Complexes: Synthesis, Biological Activity and Their Interaction with DNA and Albumins