Mitotic chromosomes are chromatin networks without a mechanically contiguous protein scaffold
AUTOR(ES)
Poirier, Michael G.
FONTE
National Academy of Sciences
RESUMO
Isolated newt (Notophthalmus viridescens) chromosomes were studied by using micromechanical force measurement during nuclease digestion. Micrococcal nuclease and short-recognition-sequence blunt-cutting restriction enzymes first remove the native elastic response of, and then to go on to completely disintegrate, single metaphase newt chromosomes. These experiments rule out the possibility that the mitotic chromosome is based on a mechanically contiguous internal non-DNA (e.g., protein) “scaffold”; instead, the mechanical integrity of the metaphase chromosome is due to chromatin itself. Blunt-cutting restriction enzymes with longer recognition sequences only partially disassemble mitotic chromosomes and indicate that chromatin in metaphase chromosomes is constrained by isolated chromatin-crosslinking elements spaced by ≈15 kb.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=137727Documentos Relacionados
- Isolation of a protein scaffold from mitotic HeLa cell chromosomes
- Bovine Papillomavirus Type 1 Genomes and the E2 Transactivator Protein Are Closely Associated with Mitotic Chromatin
- The myc proteins are not associated with chromatin in mitotic cells.
- Association of Chromatin Proteins High Mobility Group Box (HMGB) 1 and HMGB2 with Mitotic Chromosomes
- A Bromodomain Protein, MCAP, Associates with Mitotic Chromosomes and Affects G2-to-M Transition