Mielopoese em camundongos geneticamente selecionados para alta ou baixa reatividade inflamatória aguda. / Myelopoiesis in mice genetically selected for high or low acute inflammatory response.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

Mice AIRmax and AIRmin exhibit significant differences in the average number of migrating leukocytes and in the protein content of inflammatory exudate produced by polyacrylamide particles. This higher inflammatory capacity of the AIRmax mice is due to three convergent factors: higher local production of chemotactic factors, increased resistance of locally infiltrated neutrophils to spontaneous apoptosis and larger production of mature neutrophils by the bone marrow. Thus, considering the differential capacity of leukocyte production between AIRmax and AIRmin mice, we are studying comparatively the myelocytic differentiation process in vitro. We verified that the BM cells of AIRmax mice showed higher proliferation levels. In addition by qPCR technique it was verified a larger expression of genes involved in the initial stages of myelopoiesis in the AIRmax BM cultures. The analysis of BM cellular protein content by 2D gel electrophoresis revealed quantitative and qualitative differences between two mouse lines.

ASSUNTO(S)

hematopoiese diferenciação celular bone marrow mice differentiation cellular medula óssea neutrophils hematopoiesis macrophage neutrófilos macrófagos camundongos

ACESSO AO ARTIGO

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