Microbial Degradation of Corrinoids VI. Reduction of Hydroxocobalamin by Cell-free Particles from Pseudomonas rubescens

AUTOR(ES)

Hufham, J. B.

RESUMO

Cell-free particles from Pseudomonas rubescens have been shown to reduce hydroxocobalamin to vitamin B12r. The particles are unable to reduce the B12r to B12s. The reduction of hydroxocobalamin is dependent upon reduced nicotinamide adenine dinucleotide and is stimulated by flavin adenine dinucleotide. Cobinamide and diaquocobinamide were reduced at 25 and 10%, respectively, of the rate of hydroxocobalamin. Cyanocobalamin, coenzyme B12, pseudovitamin B12, and diaquopseudocobalamin were not reduced. Reduced nicotinamide adenine dinucleotide phosphate and flavin mononucleotide were not active. Diaphorase and xanthine oxidase activity were not present in the particulate fraction.

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