Métodos alternativos para análises da capacidade de ligação dos espermatozóides caprinos / Alternatives methods for analysis of the goat sperm binding Capacity

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

23/02/2010

RESUMO

This study aimed to carry out the analysis using the simple binding of goat sperm to bovine oocytes and to the perivitelline membrane of the hen s egg yolk compared to binding to the goat oocytes, looking for an alternative to predict the buck semen quality without losing the precision and accuracy of their own oocytes. Sixteen doses of goat semen were collected from 04 breed bucks, 2 Saanen and 2 Alpine via artificial vagina, using an estrus female as dummy. The physical aspects of the semen (volume, sperm whirlwind, force and motility) and sperm concentration were evaluated. Three groups were formed, and 20 μL of semen from the same collection were thawed at a concentration of 5 x 106 spermatozoon/mL and used. In the 1st group goat oocytes (LIGCAP) and in the 2nd group bovine oocytes (LIGBOV) were inseminated and in the 3rd one the perivitelline membrane of the hen s egg yolk (MPVGOD) were used. Two others groups were formed, in the first and in the second one 16 doses of fresh (MPVGOF) and thawed (MPVGOD) semen from the same collection were used, respectively, to inseminate. Semen samples from both groups were used to inseminate perivitelline membrane from chicken egg yolk. The imposed treatment did not affect the animals variables studied (P>0.05). The average volume of semen collected was 1.1 0.15 mL. The average sperm concentration observed was:1.94 0.21 x 109. The average sperm whirlwind was 3.6 0.23. The sperm motility was 77.5 1.37 and 32.2 1.37 for fresh and thawed sperm, respectively. No differences (P>0.05) among the binding assays using the bovine oocytes or hens egg yolk in relation to the goat oocytes were showned. The binding assay using fresh and thawed semen did not differ between themselves. It is concluded that, both the MPVGO and the LIGBOV may be used to replace LIGCAP. The fresh or thawed semen may be used to evaluate the binding capacity of the semen through the MPVGO without compromise the results.

ASSUNTO(S)

pelvimetria novilhas puberdade producao animal puberty heifers pelvimetry

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