Light-emitting diode effects on combined decellularization of tracheae. A novel approach to obtain biological scaffolds
AUTOR(ES)
Evaristo, Thaiane Cristine, CruzAlves, Flávia Cilene Maciel da, Moroz, Andrei, Mion, Woner, Acorci-Valério, Michele Janegitz, Felisbino, Sérgio Luis, Rossi-Ferreira, Rosana, Ruiz Júnior, Raul Lopes, Deffune, Elenice
FONTE
Acta Cir. Bras.
DATA DE PUBLICAÇÃO
2014-08
RESUMO
PURPOSE: To obtain a decellularized tracheal scaffold associating traditional approaches with the novel light-emitting diode (LED) proposal.METHODS:This study was performed with New Zealand adult rabbits weighing 3.0 - 4.0 kg. Different protocols (22) were used combining physical (agitation and LED irradiation), chemical (SDS and Triton X-100 detergents), and enzymatic methods (DNase and RNase).RESULTS:Generally, the cells surrounding soft tissues were successfully removed, but none protocol removed cells from the tracheal cartilage. However, longer protocols were more effective. The cost-benefits relation of the enzymatic processes was not favorable. It was possible to find out that the cartilaginous tissue submitted to the irradiation with LED 630nm and 475 nm showed an increased number of gaps without cells, but several cells were observed to be still present.CONCLUSION:The light-emitting diode is a promising tool for decellularization of soft tissues.
Documentos Relacionados
- Infrared Light-Emitting Diode (LED) Effects on Orthodontic Tooth Movement
- Errata: Infrared Light-Emitting Diode (LED) Effects on Orthodontic Tooth Movement
- Errata: Infrared Light-Emitting Diode (LED) Effects on Orthodontic Tooth Movement
- Crossreactivity between the light-emitting systems of distantly related organisms: Novel type of light-emitting compound
- Reproducibility of the visual evoked potential using a light-emitting diode stimulator.