Liberação do anion superoxido por fagocitos de pacientes com lupus eritematoso sistemico juvenil / Superoxide release by phagocytes from juvenile systemic lupus erythematosus patients

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

Systemic lupus erythematosus (SLE) is an autoimmune inflammatory disease with tissular injury due to immune complex deposition. The superoxide anion (SO) released by phagocytes during the inflammatory process is dangerous to the tissues. Studies performed with SLE adults revealed that SO liberation is correlated with the inflammatory activity. There are no studies with patients with juvenile SLE (JSLE). The main objective of this analytic study was to analyze the spontaneous and stimulated liberation of SO by the polimorphonucleates (PMN) and monocytes (MO) of 23 patients (3M:20F) with JSLE according to Tan criteria (1982). Such liberation of SO was expressed in nmol by 106 cells, in six different times of incubation. A first analysis compared the JSLE group with a control group of 35 healthy people. A second analysis compared the control group with two subgroups of the JSLE group, which was classified by SLEDAI, in one subgroup inactive (score <5 ) (15 patients) and another subgroup with activity (score >5) ( 8 patients). Variance analysis, Mann-Whitney and Bonferroni test were used with 5% of significance level. The results showed that in the spontaneous MO assay there was less liberation of SO in the JSLE group than in the control group. In the PMN stimulated assay there was more SO liberation in the JSLE group than in the control. There was no significant difference in SO liberation between the two subgroups of JSLE in any assay. The lower liberation of SO by the JSLE group in the spontaneous assays could be due to the anti-inflammatory and immunosupressor actions of the treatment drugs. The significative higher liberation of SO in the assays of the stimulated PMN, mainly in the initial moments, the so called ?respiratory burst? could be attributed to the inflammatory state of the phagocytes, that due to the JSLE, would be in a high excitatory level. The stimulated PMN of children with JSLE released more SO when compared with controls

ASSUNTO(S)

radicais livres adolescentes lupus erythematosus fagocitos lupus eritematoso sistemico free radicals infants adolescent systemic phagocytes infancia

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