Key for protein coding sequences identification: computer analysis of codon strategy.
AUTOR(ES)
Rodier, F
RESUMO
The signal qualifying an AUG or GUG as an initiator in mRNAs processed by E. coli ribosomes is not found to be a systematic, literal homology sequence. In contrast, stability analysis reveals that initiators always occur within nucleic acid domains of low stability, for which a high A/U content is observed. Since no aminoacid selection pressure can be detected at N-termini of the proteins, the A/U enrichment results from a biased usage of the code degeneracy. A computer analysis is presented which allows easy detection of the codon strategy. N-terminal codons carry rather systematically A or U in third position, which suggests a mechanism for translation initiation and helps to detect protein coding sequences in sequenced DNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=326141Documentos Relacionados
- A backtranslation method based on codon usage strategy.
- The codon preference plot: graphic analysis of protein coding sequences and prediction of gene expression.
- Computer programs for the characterization of protein coding genes.
- Theoretical analysis of library screening using a N-dimensional pooling strategy.
- Codon preference and its use in identifying protein coding regions in long DNA sequences