Isolation and Characterization of Organelles from Soybean Suspension Cultures 1
AUTOR(ES)
Moore, T. S.
RESUMO
Whole homogenates from cells of Glycine max grown in suspension culture were centrifuged on linear sucrose gradients. Assays for marker enzymes showed that distinct peaks enriched in particular organelles were separated as follows: endoplasmic reticulum (density 1.10 g/cm3, NADH-cytochrome-c reductase), Golgi membranes (density 1.12 g/cm3, inosine diphosphatase), mitochondria (density 1.18—1.19 g/cm3, fumarase, cytochrome oxidase) and microbodies (density 1.21—1.23 g/cm3, catalase). In cells which had ceased to grow (stationary phase) only a single symmetrical catalase peak at density 1.23 g/cm3 was observed on the sucrose gradient. During the phase of cell division and expansion a minor particulate catalase component of lighter density was present; its possible significance is discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=541375Documentos Relacionados
- Vacuoles from Sugarcane Suspension Cultures 1: I. ISOLATION AND PARTIAL CHARACTERIZATION
- Replication of DNA by Nuclei Isolated from Soybean Suspension Cultures 1
- An Extracellular Macromolecular Complex from the Surface of Soybean Suspension Cultures 1
- Secretion of active recombinant phytase from soybean cell-suspension cultures.
- Vacuoles from Sugarcane Suspension Cultures 1: II. CHARACTERIZATION OF SUGAR UPTAKE
