Isolation and Characterization of a Temperature-Sensitive Circadian Clock Mutant of Neurospora Crassa
AUTOR(ES)
Morgan, L. W.
RESUMO
A new circadian clock mutant has been isolated in Neurospora crassa. This new mutation, called period-6 (prd-6), has two features novel to known clock mutations. First, the mutation is temperature sensitive. At restrictive temperatures (above 21°) the mutation shortens circadian period length from a wild-type value of 21.5 hr to 18 hr. At permissive temperatures (below 21°) the mutant has a 20.5-hr period length close to that of the wild-type strain. Second, the prd-6 mutation is epistatic to the previously isolated clock mutation period-2 (prd-2). This epistasis is unusual in that the prd-2 prd-6 double mutant strain has an 18-hr period length at both the restrictive and permissive temperatures. That is, the temperature-sensitive aspect of the phenotype of the prd-6 strain is lost in the prd-2 prd-6 double mutant strain. This suggests that the gene products of the prd-2 and prd-6 loci may interact physically and that the presence of a normal prd-2(+) protein is required for low temperature to ``rescue'' the prd-6 mutant phenotype. These results, combined with our recent finding that prd-2 and some alleles of the frq gene show genetic synergy, suggest that it may be possible to establish a more comprehensive model of the Neurospora circadian clock.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1207994Documentos Relacionados
- Isolation and Characterization of Temperature-Sensitive Respiratory Mutants of NEUROSPORA CRASSA
- Neurospora crassa temperature-sensitive mutant apparently defective in protein synthesis.
- Isolation and characterization of a temperature-sensitive mutant of avian myeloblastosis virus.
- Temperature-Sensitive Pleiotropic Revertants from a Mutant in the AROM Gene Cluster of NEUROSPORA CRASSA
- Isolation and characterization of a temperature-sensitive dnaK mutant of Escherichia coli B.
