Isolation and characterization of a species-specific DNA probe for Candida albicans.
AUTOR(ES)
Oren, I
RESUMO
As a model for the isolation of species-specific sequences of DNA, we isolated and characterized a species-specific DNA fragment from the Candida albicans genome. We used a series of differential colony hybridization experiments, in which a C. albicans genomic library was hybridized with genomic DNA probes from related organisms to minimize the number of potentially specific C. albicans DNA fragments to be tested. Six clones were tested by dot blot analysis, and one of them, a 1348 bp EcoRI DNA fragment, was confirmed as specific for C. albicans. This species-specific fragment could be utilized as a DNA probe for rapid, sensitive, and specific diagnosis of C. albicans DNA in clinical specimens.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=332528Documentos Relacionados
- Isolation and characterization of a species-specific DNA fragment for detection of Candida albicans by polymerase chain reaction.
- Isolation and Characterization of a Species-Specific DNA Fragment for Identification of Candida (Torulopsis) glabrata by PCR
- A species-specific DNA probe for the detection of Mycoplasma gallisepticum.
- Molecular cloning of the secretory acid proteinase gene from Candida albicans and its use as a species-specific probe.
- Rapid Identification of Candida Species with Species-Specific DNA Probes