Isolation and characterization of a human cementocyte-like cell line, HCY-23
AUTOR(ES)
ALMEIDA, Amanda Bandeira de
FONTE
Braz. oral res.
DATA DE PUBLICAÇÃO
15/08/2019
RESUMO
Abstract Cementum is the mineralized tissue covering the tooth root that functions in tooth attachment and post-eruptive adjustment of tooth position. It has been reported to be highly similar to bone in several respects but remains poorly understood in terms of development and regeneration. Here, we investigate whether cementocytes, the residing cells in cellular cementum, have the potential to be protagonist in cementum homeostasis, responding to endocrine signals and directing local cementum metabolism. Cells from healthy erupted human teeth were isolated using sequential collagenase/EDTA digestions, and maintained in standard cell culture conditions. A cementocyte-like cell line was cloned (HCY-23, for human cementocyte clone 23), which presented a cementocyte compatible gene expression signature, including the expression of dentin matrix protein 1 ( DMP1 ), sclerostin ( SOST ), and E11/gp38/podoplanin ( E11 ). In contrast, these cells did not express the odontoblast/dentin marker dentin sialoprotein ( DSPP ). HCY-23 cells produced mineral-like nodules in vitro under differentiation conditions, and were highly responsive to inorganic phosphate (Pi). Within the limits of the present study, it can be concluded that cementocytes are phosphate-responsive cells, and have the potential do play a key role in periodontal homeostasis and regeneration.
Documentos Relacionados
- Conventional cytogenetic characterization of a new cell line, ACP01, established from a primary human gastric tumor
- DNA repair in human promyelocytic cell line, HL-60.
- The establishment and characterization of the first canine hepatocellular carcinoma cell line, which resembles human oncogenic expression patterns
- Isolation, immortalization, and characterization of a human breast epithelial cell line with stem cell properties
- Isolation and preliminary characterization of the transforming gene of a human neuroblastoma cell line.