Influência da rugosidade e energia livre de superfície de materiais para próteses removíveis a base de polimetilmetacrilato nas propriedades do biofilme de Candida albicans / Influence of the roughness and free energy surfasse of the materials for removable prosthesis the base of polymethymethacrylate in the properties of biofilm of Candida albicans
AUTOR(ES)
Cristiane Maria Brasil Leal
FONTE
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia
DATA DE PUBLICAÇÃO
29/07/2011
RESUMO
The properties of surface of the resins and reliners base of polymethymethacrylate used in removable dental prosthesis can interfere in the adhesion, colonization and formation of biofilm of Candida spp., microorganism associated to the prosthetic stomatitis. The objective of this research was to evaluate the influence of the roughness and the free energy of surface of the resin the base of polymethymethacrylate and a rigid reliner in the adhesion and structure of biofilms of C. albicans. Discs of the termopolymerized acrylic resin and rigid reliner to the base of polymethymethacrylate had been confectioned in the dimensions 10 x 2 mm. The differents roughness of surface of the specimens were obtained by means of different finishing/polishing. The free energy of surface varied for the formation of pellicle of saliva or serum or the combination of both on the specimens. After the measurement the roughness and the free energy of surface, the specimens were divided randomly in accordance to the experimental groups. Biofilms of Candida albicans ATCC 90028 were formed on the surfaces and evaluated for cell count, bioactivity and architecture (bio-volume, average thickness and average roughness) in the adhesion phases and after 48 hours. The data were analyzed using analysis of variance with two criteria followed by the Tukey test. The significance level was p=0,05. The results showed that even with significant differences (p<0.001) between the values of roughness, it did not reflect in the parameters evaluated of biofilm of C. albicans in 48 hours. There was no significant difference in the values of the free energy of surface; however, the difference of the pellicles caused significant alterations of biofilm of C. albicans in the adhesion phase and 48 hours (p>0,05). It can be concluded that the alteration of the roughness of surface don´t affect the structure of biofilm of C. albicans of 48 hours, however, the alteration of the free energy of surface by deposition of salivary and/or serum proteins modifies the structure of biofilm in the adhesion phases and 48 hours.
ASSUNTO(S)
rugosidade de superfície candida albicans biofilme surface roughness candida albicans biofilms
ACESSO AO ARTIGO
http://libdigi.unicamp.br/document/?code=000812279Documentos Relacionados
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