InfluÃncia do Ãleo de algodÃo, glicose e extrato de levedura Na produÃÃo de biossurfactante por uma nova linhagem de Candida glabrata

AUTOR(ES)

Juliana Moura de Luna

DATA DE PUBLICAÇÃO

2006

RESUMO

The production of surfactants of microbiologic origin has been intensively investigated during the last few years due their biotechnological potential and applications in different industrial sectors. Thus, the aim of the present work was to produce surfactants by a new strain of Candida glabrata UCP 1002 isolalated from mangrove sediments. Cotton seed oil, glucose and yeast extract were used as substrates. The growth kinetics, biossurfactant production, biosurfactant properties, isolation, characterization and application in oil removal were studied. A factorial design was first conducted and the best conditions for n-hexadecane and cotton seed oil emulsification were found in the central point of the experimental design (mineral medium containing 7.5 % cotton seed oil, 5.0 % glucose and 0.3% yeast extract). The surface tension of the cell-free broth containing the biosurfactant produced under the conditions of the central point, after 144 hours of cultivation at 150 rpm, revealed the ability of the biosurfactant in reducing the surface tension of water from 70 mN/m to 31 mN/m. The biopolymer produced showed stability under extreme conditions of pH and temperature and in the presence of high salt concentrations. The yield of biossurfactant produced was 10 g/L, with a critical micelar concentration of 2.5%. The emulsification activity of n-hexadecane by the cell-free broth was totally recovered by the isolated biosurfactant. The isolated biosurfactant was able to recover 84% of the motor oil from contaminated sand. The characterization of the biosurfactant showed the presence of 45 % protein, 20% lipid and 10 % carbohydrate. The results obtained for the biosurfactant produced by a new Candida glabrata strain cultivated under conditions described above showed attractive properties of the biopolymer, specially in oils removal

ASSUNTO(S)

ciencias biologicas olÃo de algodÃo, glicose e extreato de levedura biossurfactantes microbiologia aplicada gandida grabrata isolada de mangue

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