Induction of Antiviral Antibodies by DNA Immunization Requires neither Perforin-Mediated nor CD8+-T-Cell-Mediated Lysis of Antigen-Expressing Cells†

AUTOR(ES)

Hassett, Daniel E.

FONTE

American Society for Microbiology

RESUMO

DNA immunization induces antibodies to the encoded protein, which indicates that the protein must gain access to the extracellular milieu, allowing it to interact with naïve B lymphocytes. It has been suggested that antigen release may be effected by cytotoxic-T-lymphocyte-mediated lysis of transfected antigen-expressing cells; this might be particularly important for the induction of responses to a noncytopathic, cytosolic protein. Here we show that the induction of antibody responses to one such DNA-encoded protein required neither perforin nor CD8+ T cells. In addition, there was no skewing of the immunoglobulin G isotypes in the absence of perforin.

Documentos Relacionados

• HLA Compatibility Requirements for CD8+-T-Cell-Mediated Suppression of Human Immunodeficiency Virus Replication
• Myonuclear apoptosis in dystrophic mdx muscle occurs by perforin-mediated cytotoxicity.
• Borna Disease Virus Nucleoprotein (p40) Is a Major Target for CD8+-T-Cell-Mediated Immune Response
• Borna Disease Virus Nucleoprotein (p40) Is a Major Target for CD8+-T-Cell-Mediated Immune Response
• Interleukin 21 up-regulates perforin-mediated cytotoxic activity of human intra-epithelial lymphocytes