Increased sensitivity of acid-fast smears.
AUTOR(ES)
Rickman, T W
RESUMO
Evaluation of the centrifuges used in the mycobacteriology laboratory indicated a failure to develop sufficient relative centrifugal force for optimal concentration of acid-fast bacilli. Retrospective analysis of 14,509 specimens received between 1 July 1973 and 30 June 1975 and sedimented at 1,260 x g relative centrifugal force revealed a positive smear rate of 1.8% and a positive culture rate of 7.1%, with a correlation between positive paired smears and cultures of 25.2%. After increasing the relative centrifugal force from 1,260 x g to 3,800 x g at maximum radius, the positive smear and culture rates were 9.6 and 11.6%, respectively, with a correlation between positive paired smears and cultures of 82.4%. The sensitivity of acid-fast smears is directly related to the relative centrifugal force achieved while concentrating the specimen by centrifugation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=273472Documentos Relacionados
- Preparation of Sputum Smears for Acid-Fast Microscopy
- Preparation of acid-fast microscopy smears for proficiency testing and quality control.
- Evaluation of sputum smears concentrated by cytocentrifugation for detection of acid-fast bacilli.
- Are Three Sputum Acid-Fast Bacillus Smears Necessary for Discontinuing Tuberculosis Isolation?
- Evaluation of the Acid-Fast Smear