Incidence and transferability of antibiotic resistance in the enteric bacteria isolated from hospital wastewater

AUTOR(ES)

Alam, Mohammad Zubair, Aqil, Farrukh, Ahmad, Iqbal, Ahmad, Shamim

FONTE

Braz. J. Microbiol.

DATA DE PUBLICAÇÃO

2013-09

RESUMO

This study reports the occurrence of antibiotic resistance and production of β-lactamases including extended spectrum beta-lactamases (ESβL) in enteric bacteria isolated from hospital wastewater. Among sixty-nine isolates, tested for antibiotic sensitivity, 73.9% strains were resistant to ampicillin followed by nalidixic acid (72.5%), penicillin (63.8%), co-trimoxazole (55.1%), norfloxacin (53.6%), methicillin (52.7%), cefuroxime (39.1%), cefotaxime (23.2%) and cefixime (20.3%). Resistance to streptomycin, chloramphenicol, nitrofurantoin, tetracycline, and doxycycline was recorded in less than 13% of the strains. The minimum inhibitory concentration (MIC) showed a high level of resistance (800-1600 µg/mL) to one or more antibiotics. Sixty three (91%) isolates produced β-lactamases as determined by rapid iodometric test. Multiple antibiotic resistances were noted in both among ESβL and non-ESβL producers. The β-lactamases hydrolyzed multiple substrates including penicillin (78.8% isolates), ampicillin (62.3%), cefodroxil (52.2%), cefotoxime (21.7%) and cefuroxime (18.8%). Fifteen isolates producing ESβLs were found multidrug resistant. Four ESβL producing isolates could transfer their R-plasmid to the recipient strain E. coli K-12 with conjugation frequency ranging from 7.0 x 10-3 to 8.8 x 10-4. The findings indicated that ESβL producing enteric bacteria are common in the waste water. Such isolates may disseminate the multiple antibiotic resistance traits among bacterial community through genetic exchange mechanisms and thus requires immediate attention.

Documentos Relacionados

• Incidence of Virulence Factors and Antibiotic Resistance among Enterococci Isolated from Food
• Effect of Antibiotic Treatment on the Incidence of Infectious Drug Resistance Among Intestinal Lactose-Fermenting Bacteria Isolated from Burn Patients
• Antibiotic resistance of gram-negative enteric bacteria from pigs in three herds with different histories of antibiotic exposure.
• Long-Term Shifts in Patterns of Antibiotic Resistance in Enteric Bacteria
• Antibiotic resistance in lactic acid bacteria isolated from some pharmaceutical and dairy products