In vitro Callus Induction and Plant Regeneration of Celosia argentea- An Important Medicinal Plant
AUTOR(ES)
Bakar, Dalilah Abu, Ahmed, Bakrudeen Ali, Taha, Rosna Mat
FONTE
Braz. arch. biol. technol.
DATA DE PUBLICAÇÃO
2014-12
RESUMO
Celosia argentea (Var.) cristata (Amaranthaceae) is a widely cultivated ornamental plant, which has antibacterial, astringent, haemostatic, hypertensive, ophthalmic, and parasitic significance. This study describes a protocol for in vitro callus induction and plant regeneration from leaf and stem explants of C. argentea using Murashige and Skoog (MS) medium. Callus culture was initiated and established from seedling, leaf, and stem explants. Explants were cultured on MS medium supplemented with auxin alone (0.5 mg/L Naphthaleneacetic acid (NAA), 2, 4-Dicholorophenoxyacetic acid (2, 4-D). Green and red compact callus (98%) were induced using MS medium supplemented with 0.5 mg/L NAA and 1.0 mg/L Benzyladenine (BA). Two different concentrations (1.0 mg/L BA + 0.5 mg/L NAA and 1.0 mg/L BA + 1.0 mg/L NAA) successfully induced plant regeneration with multiple shoots (1.5 and 0.9 shoots per explants, respectively). Successful shoots were transferred to rooting medium supplemented with 1.0 mg/L Indole-3-acetic acid (IAA) (80%) at 35thday. Acclimatization was done, which resulted in 90% of the plantlets surviving in garden soil. This protocol could be used to micropropagate C. argentea for conservation, commercial natural product production. The high frequency of callus indicated potential of C. argentea for secondary metabolite production (celosin) in pharmaceutical industry.
Documentos Relacionados
- In vitro callus induction and micropropagation of Thymus persicus (Lamiaceae), an endangered medicinal plant
- Somatic embryogenesis and plant regeneration in Wedelia calendulacea Less. an endangered medicinal plant
- Identificação molecular de um fitoplasma associado à malformação das folhas das ornamentais Celosia argentea L. e Celosia spicata L.
- Review on research of the phytochemistry and pharmacological activities of Celosia argentea
- Somatic embryogenesis and plant regeneration from callus cultures of Cleome rosea Vahl