In TH2 cells the Il4 gene has a series of accessibility states associated with distinctive probabilities of IL-4 production

AUTOR(ES)

Guo, Liying

FONTE

National Academy of Sciences

RESUMO

TH2 clones may produce very variable amounts of IL-4. Among six TH2 clones prepared from homozygous or heterozygous mice in which Gfp replaced the first exon of Il4, a range of patterns of CpG methylation in the Il4/Il13 locus was observed correlating with the degree of expression of IL-4 or green fluorescence protein. Patterns of histone acetylation also showed differences between “high” and “low” TH2 clones. These results indicate that in TH2 cells the Il4 locus may display variable patterns of chromatin accessibility associated with distinct degrees of IL-4 expression. This finding suggests a regulation of IL-4 expression keyed to the function of this cytokine in cell/cell interactions and in the regulation of threshold responses.

Documentos Relacionados

• Polarized Th2 like cells, in the absence of Th0 cells, are responsible for lymphocyte produced IL-4 in high IgE-producer schistosomiasis patients
• Th2 responses induced by epicutaneous or inhalational protein exposure are differentially dependent on IL-4
• IL-4 dependent alternatively-activated macrophages have a distinctive in vivo gene expression phenotype
• Oral immunization of interleukin-4 (IL-4) knockout mice with a recombinant Salmonella strain or cholera toxin reveals that CD4+ Th2 cells producing IL-6 and IL-10 are associated with mucosal immunoglobulin A responses.
• An IL-4-independent and CD25-mediated function of c-maf in promoting the production of Th2 cytokines