Improvements to solid phase phosphotriester synthesis of deoxyoligonucleotides.
AUTOR(ES)
Patel, T P
RESUMO
A solution of benzenesulphonic acid (3%, w/v) in a dimethylformamide and dichloromethane mixture (9:1, v/v) is shown to be a very effective reagent for the detritylation of deoxyoligonucleotides attached to a solid support. The levels of depurination with this reagent were lower than those observed with other reagents such as trichloroacetic acid. Coupling reactions are described using above ambient temperatures with no detectable increase in side products. Both procedures have been successfully incorporated into an automated system, which can compete with the rate of synthesis by the phosphite approach.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=320910Documentos Relacionados
- Solid phase synthesis of polynucleotides. VIII. Synthesis of mixed oligodeoxyribonucleotides by the phosphotriester solid phase method.
- Hybridization triggered cross-linking of deoxyoligonucleotides.
- Solid phase phosphotriester synthesis of large oligodeoxyribonucleotides on a polyamide support.
- Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates.
- Silica gel: an improved support for the solid-phase phosphotriester synthesis of oligonucleotides.