Impact of Virioplankton on Archaeal and Bacterial Community Richness as Assessed in Seawater Batch Cultures
AUTOR(ES)
Winter, Christian
FONTE
American Society for Microbiology
RESUMO
During cruises in the tropical Atlantic Ocean (January to February 2000) and the southern North Sea (December 2000), experiments were conducted to monitor the impact of virioplankton on archaeal and bacterial community richness. Prokaryotic cells equivalent to 10 to 100% of the in situ abundance were inoculated into virus-free seawater, and viruses equivalent to 35 to 360% of the in situ abundance were added. Batch cultures with microwave-inactivated viruses and without viruses served as controls. The apparent richness of archaeal and bacterial communities was determined by terminal restriction fragment length polymorphism (T-RFLP) analysis of PCR-amplified 16S rRNA gene fragments. Although the estimated richness of the prokaryotic communities generally was greatly reduced within the first 24 h of incubation due to confinement, the effects of virus amendment were detected at the level of individual operational taxonomic units (OTUs) in the T-RFLP patterns of both groups, Archaea and Bacteria. One group of OTUs was detected in the control samples but was absent from the virus-treated samples. This negative response of OTUs to virus amendment probably was caused by viral lysis. Additionally, we found OTUs not responding to the amendments, and several OTUs exhibited variable responses to the addition of inactive or active viruses. Therefore, we conclude that individual members of pelagic archaeal and bacterial communities can be differently affected by the presence of virioplankton.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=348926Documentos Relacionados
- Influence of temperature and substrate concentration on bacterial growth yield in Seine River water batch cultures.
- Impact of Protozoan Grazing on Bacterial Community Structure in Soil Microcosms
- Impact of Dilution on Microbial Community Structure and Functional Potential: Comparison of Numerical Simulations and Batch Culture Experiments
- Dimethyl Sulfide Production from Dimethylsulfoniopropionate in Coastal Seawater Samples and Bacterial Cultures †
- Bacterial response to siderophore and quorum-sensing chemical signals in the seawater microbial community