Immune effector cell activity in canines: failure to demonstrate genetic restriction in direct antiviral cytotoxicity.
Ho, C K
Experiments were undertaken to establish whether the cytotoxic activity of canine immune effector cells against viral antigens was affected by the genotype of the target cell (genetic restriction). Puppies from three different breeds were infected with vaccinia virus, and the peripheral blood leukocytes were collected at various times for measurement of their cytotoxicity against autologous and heterologous vaccinia virus-infected and uninfected skin fibroblasts. In all cases cytotoxicity only occurred against virus-infected targets, and there was no consistent evidence of preferential killing of autologous targets. Several indirect approaches were used to demonstrate that direct, presumably T cell, cytotoxicity was being measured rather than antibody-dependent cell cytotoxicity. On the basis of the evidence from cross mixed-lymphocyte assays and cell-mediated lympholysis assays, the dogs were shown not be be identical with respect to their histocompatibility antigens. The significance of our findings to the phenomenon of genetic restriction as observed for mouse-derived immune effector cells is briefly discussed.
ACESSO AO ARTIGOhttp://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=414041
- Behaviour of effector cells, synovial fluids, and sera from rheumatoid arthritis patients in antibody-dependent cell-mediated cytotoxicity.
- Liver macrophages (Kupffer cells) as cytotoxic effector cells in extracellular and intracellular cytotoxicity.
- Orthodontic traction of impacted canines: Concepts and clinical application
- Epithelial cell polarity affects susceptibility to Pseudomonas aeruginosa invasion and cytotoxicity.
- Role of thymidylate synthetase activity in development of methotrexate cytotoxicity.