Immobilization of laccase on hybrid layered double hydroxide
AUTOR(ES)
Camacho Córdova, David Isidoro, Morales Borges, Rogelio, Arizaga, Gregorio Guadalupe Carbajal, Wypych, Fernando, Krieger, Nádia
FONTE
Química Nova
DATA DE PUBLICAÇÃO
2009
RESUMO
Crystals of Mg/Al layered double hydroxide were synthesized by alkaline precipitation and treated in an aqueous solution of glutamic acid. The glutamate ions were not intercalated into the interlayer space, but were detected in the material by Fourier transform infrared spectroscopy, suggesting that only the external surfaces of crystals were modified with glutamate ions. The resulting hybrid material was tested as a support for immobilization of the enzyme laccase (Myceliophthora thermophila). The immobilized enzyme preparation was characterized by electronic paramagnetic resonance spectroscopy and by assays of catalytic activity. The activity of the immobilized laccase was 97% of the activity in the free enzyme. Layered double hydroxide is a suitable support for use in remediation of soil studies.
Documentos Relacionados
- Nickel-Cerium Layered Double Hydroxide as Electrocatalyst for Glycerol Oxidation
- New hybrid material based on layered double hydroxides and biogenic silver nanoparticles: antimicrobial activity and cytotoxic effect
- Ferulate Anion Intercalated into Zn/Al Layered Double Hydroxide: A Promising Intercalation Compound for Inhibition of Leishmania (L.) amazonensis
- Determination of mesalamine by spectrofluorometry in human serum after solid-phase extraction with Ni-Al layered double hydroxide as a nanosorbent
- Estudo de metalofármacos antiinflamatórios de cobre e dos materiais híbridos resultantes de suas imobilizações no hidróxido duplo lamelar hidrotalcita: síntese, caracterização e avaliação da atividade farmacológica