Identification of candidate antigens from adult stages of Toxocara canis for the serodiagnosis of human toxocariasis
AUTOR(ES)
Peixoto, Patrícia Longuinhos, Nascimento, Evaldo, Cançado, Guilherme Grossi Lopes, Miranda, Rodrigo Rodrigues Cambraia de, Rocha, Regina Lunardi, Araújo, Ricardo Nascimento, Fujiwara, Ricardo Toshio
FONTE
Memórias do Instituto Oswaldo Cruz
DATA DE PUBLICAÇÃO
2011-03
RESUMO
In the present work, we identified adult Toxocara canis antigens through sodium dodecyl sulfate-polyacrylamide gel electrophoresis for potential use in human toxocariasis immunodiagnosis. The sensitivity and specificity of several semi-purified antigens, as well as their cross-reactivity with other parasitic infections, were assessed by IgM and IgG-enzime linked immunosorbent assay. Whilst we found that the crude extract of the parasite presented limited sensitivity, specificity and high cross-reactivity against other parasites, we identified 42, 58, 68 and 97-kDa semi-purified antigens as the most promising candidates for immunodiagnosis. Moreover, the 58 and 68-kDa antigens presented the lowest IgM cross-reactivity. When tested as a combination, a mixture of the 58 and 68-kDa antigens presented 100% sensitivity and specificity, as well as minor cross-reactivity. Although the combination of the 42, 58, 68 and 97-kDa antigens presented 100% sensitivity at a dilution of 1:40, the low specificity and high cross-reactivity observed suggested a limited use for diagnostic purposes. Our data suggested that the 58 and 68-kDa antigens might be most suitable for the immunodiagnosis of human toxocariasis.
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