Identification and characterization of a new transcriptional termination factor from Escherichia coli.

AUTOR(ES)

Briat, J F

RESUMO

We have identified and partially purified an activity from Escherichia coli that enhances transcription termination at the bacteriophage T7 early terminator when cloned on the plasmid pAR1707. The factor also causes the transcript to be terminated at a site several nucleotides earlier than in its absence. The resulting 3' OH ends of the transcripts are identical to those found in vivo by S1 nuclease mapping. From this we conclude that the factor we have identified is probably responsible for determination of the 3' OH ends of T7 RNA transcripts in vivo. This factor does not act by processing a preformed RNA transcript, nor is it replaced by rho protein or nusA or nusB proteins. Therefore, it appears to be a new transcription termination factor, and we have designated it "tau factor." Elucidation of its role in transcription in E. coli will depend on its purification to homogeneity and further studies of its properties.

Documentos Relacionados

• Identification and characterization of CS20, a new putative colonization factor of enterotoxigenic Escherichia coli.
• Immunochemical identification and biological characterization of cytotoxic necrotizing factor from Escherichia coli.
• Identification and characterization of a new disulfide isomerase-like protein (DsbD) in Escherichia coli.
• Identification of FtsW and characterization of a new ftsW division mutant of Escherichia coli.
• Identification and characterization of the caiF gene encoding a potential transcriptional activator of carnitine metabolism in Escherichia coli.