Hypothalamic endoplasmic reticulum stress of overtrained mice after recovery

AUTOR(ES)

Pinto, Ana P., Oliveira, Luciana da C., Rocha, Alisson L. da, Pereira, Bruno C., Morais, Gustavo P., Vicente, Larissa Gaioto de, Moura, Leandro P., Pauli, José R., Silva, Adelino S. R. da

FONTE

Motriz: rev. educ. fis.

DATA DE PUBLICAÇÃO

02/05/2017

RESUMO

Abstract AIMS knowing the relationship between endoplasmic reticulum (ER) stress and inflammation and based on the fact that downhill running-based overtraining (OT) model increases hypothalamus levels of some pro-inflammatory cytokines, we verified the effects of three OT protocols on the levels of BiP, pIRE-1 (Ser734), pPERK (Thr981), pelF2alpha (Ser52), ATF-6 and GRP-94 proteins in the mouse hypothalamus after two weeks of recovery. METHODS the mice were randomized into control (CT), overtrained by downhill running (OTR/down), overtrained by uphill running (OTR/up) and overtrained by running without inclination (OTR) groups. After 2-week total recovery period (i.e., week 10), hypothalamus was removed and used for immunoblotting. RESULTS the OTR/down group exhibited high levels of BiP and ATF6. The other OT protocols showed higher levels of pPERK (Th981) and pelf-2alpha (Ser52) when compared with the CT group. CONCLUSION the current results suggest that after a 2-week total recovery period, the overtrained groups increased partially their ER stress protein levels, but without hypothalamic inflammation, which characterizes a physiological condition related to an adaptation mechanism.

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