HPLC-DAD and UV-Vis Spectrophotometric Methods for Methotrexate Assay in Different Biodegradable Microparticles
AUTOR(ES)
Oliveira, Alice R., Caland, Lilia B., Oliveira, Edilene G., Egito, Eryvaldo S. T., Pedrosa, Matheus F. F., Silva Júnior, Arnóbio A.
FONTE
J. Braz. Chem. Soc.
DATA DE PUBLICAÇÃO
2015-04
RESUMO
The challenge of the present work concerned the development and validation of high performance liquid chromatography (HPLC) and UV-Vis spectrophotometric methods for quantitation of methotrexate (MTX) loaded on biodegradable microparticles, composed of copolymers with different solubilities such as chitosan and poly (lactic-co-glycolic acid) (PLGA). The linearity of the analytical curves for MTX were > 0.999 (r2) and the limits of detection (LOD) were 0.014 µg mL-1 and 0.060 µg mL-1 for HPLC and UV-Vis spectrophotometric method, respectively. In addition, both methods were specific, robust, and accurate with a recovery between 89.5% and 105.5%. The method showed precision with relative standard deviations lower than 3.39% for HPLC and 2.90% for UV-Vis spectrophotometry. Statistical analysis revealed that both methods provide equivalent results, and also can be used for quality control of MTX-loaded in drug delivery systems.
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