High-pressure liquid chromatographic method for analysis of cephalosporins.
AUTOR(ES)
Signs, S A
RESUMO
A high-pressure liquid chromatographic method is described for the analysis of a wide range of cephalosporin congeners, using only three reagents for extraction and drug analysis. Plasma was treated with cold methanol-0.1 M sodium acetate to precipitate protein. Cephalosporins were resolved on a C-18 reverse-phase column, utilizing a mobile phase of various percentages of 0.01 M sodium acetate and acetonitrile-methanol. Compounds analyzed included cephalexin, cefamandole, cephalothin, cefotaxime, cefazolin, cephaloridine, cefoxitin, cefaclor, cephapirin, and cefoperazone. Each antibiotic demonstrated excellent linearity throughout the therapeutic range. The method of standard additions revealed recoveries of 93 to 101%, with detection limits ranging from 0.2 to 1.0 micrograms/ml for these drugs. Retention times ranged from 4 to 6 min. This method offers a rapid and simple means by which this group of cephalosporins may be reliably quantitated.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=179987Documentos Relacionados
- Rapid high-pressure liquid chromatographic method for analysis of phenoxymethylpenicillin in human serum.
- High-pressure liquid chromatographic method for determination of rosaramicin in humans.
- High-pressure liquid chromatographic quantitation of azlocillin.
- High-pressure liquid chromatographic method for determination of Sch 28191 in biological fluids.
- High-pressure liquid chromatographic method for determination of Sch 29482 in human serum.