Hepatitis E virus in liver and bile samples from slaughtered pigs of Brazil

AUTOR(ES)

Gardinali, Noemi Rovaris, Barry, Aline Fernandes, Otonel, Rodrigo Alejandro Arellano, Alfieri, Alice Fernandes, Alfieri, Amauri Alcindo

FONTE

Mem. Inst. Oswaldo Cruz

DATA DE PUBLICAÇÃO

2012-11

RESUMO

The objective of this study was to detect and identify hepatitis E virus (HEV) strains in liver and bile samples from slaughtered pigs in the state of Paraná, Brazil. Liver and bile samples were collected from 118 asymptomatic adult pigs at a slaughterhouse in a major Brazilian pork production area. The samples were assayed using a nested reverse transcription-polymerase chain reaction protocol with primer sets targeting open reading frames (ORF)1 and 2 of the HEV genome. HEV RNA was detected in two (1.7%) liver samples and one (0.84%) bile sample using both primers sets. The HEV strains were classified as genotype 3b on the basis of their nucleotide sequences. These data suggest that healthy pigs may be a source of HEV infection for consumers of pig liver and slaughterhouse workers in Brazil.

Documentos Relacionados

• Micronuclei formation in liver fibrosis samples from patients infected by hepatitis C virus
• Low occurrence of Hepatitis A virus in water samples from an urban area of Southern Brazil
• Development of a Combined Selection and Enrichment PCR Procedure for Clostridium botulinum Types B, E, and F and Its Use To Determine Prevalence in Fecal Samples from Slaughtered Pigs
• Detection of Hepatitis B Virus Antigens in Paraffin-embedded Liver Specimens from the Amazon Region, Brazil
• Hepatitis C virus in blood samples from volunteer donors.