Growth and Development of Tetracycline-Resistant Chlamydia suis†
AUTOR(ES)
Lenart, J.
FONTE
American Society for Microbiology
RESUMO
Tetracycline (TET) is a front-line antibiotic for the treatment of chlamydial infections in both humans and animals, and the emergence of TET-resistant (Tetr) Chlamydia is of significant clinical importance. Recently, several Tetr chlamydial strains have been isolated from swine (Sus scrofa) raised in production facilities in Nebraska. Here, the intracellular development of two Tetr strains, R19 and R27, is characterized through the use of tissue culture and immunofluorescence. The strains grow in concentrations of up to 4 μg of TET/ml, while a TET-sensitive (Tets) swine strain (S45) and a strain of the human serovar L2 (LGV-434) grow in up to 0.1 μg of TET/ml. Although inclusions form in the presence of TET, many contain large aberrant reticulate bodies (RBs) that do not differentiate into infectious elementary bodies. The percentage of inclusions containing typical developmental forms decreases with increasing TET concentrations, and at 3 μg of TET/ml 100% of inclusions contain aberrant RBs. However, upon removal of TET the aberrant RBs revert to typical RBs, and a productive developmental cycle ensues. In addition, inclusions were found that contained both C. suis R19 and Chlamydia trachomatis L2 after sequential infection, demonstrating that two biologically distinct chlamydial strains could both develop within a single inclusion.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=90631Documentos Relacionados
- Development of Tetracycline-Resistant Staphylococcus aureus in Gnotobiotic Mice
- Characterization of unusual tetracycline-resistant gram-positive bacteria.
- Isolation and Characterization of Tetracycline-Resistant Clinical Isolates of Helicobacter pylori
- Auxotypes and serogroups of tetracycline-resistant Neisseria gonorrhoeae isolated in Malaysia.
- Occurrence of tetracycline-resistant Neisseria gonorrhoeae outside the United States.
