Genotipagem , utilizando a sequencia de inserção IS6110, de cepas de Mycobacterium tuberculosis isoladas de pacientes portadores da infecção pelo HIV em Moçambique, Africa / IS6110 Polymorphism in Mycobacterium tuberculosis isolates from HIV infected patients living in Mozambique, Africa

AUTOR(ES)

Audrey Jordão Basso

DATA DE PUBLICAÇÃO

2006

RESUMO

IS6110 RFLP has been the most widely used genetic subtyping method for M. tuberculosis strains, to characterize disease outbreaks or for evolutionary genetics studies. In Mozambique, where tuberculosis exhibits a high prevalence, there is not enough information about IS6110-RFLP patterns of local M. tuberculosis strains. The description of the fingerprinting patterns obtained with this methodology can be useful locally for epidemiological purposes, and internationally to investigate the relatedness of strains isolated in Mozambique to other areas of the world. In this study, a collection of 158 isolates of M. tuberculosis strains, as identified by using hsp65-PRA, recovered from HIV-infected patients with pulmonary tuberculosis residing in Maputo, Mozambique, was genotyped. The number of IS6110 copies ranged from 1 to 18, with 21.5% of strains exhibiting less than six copies. A total of 10 clusters were found, one consisting of three strains and all the others of two strains. Isolates showing less than six bands were not included in the cluster analyses due to low discriminatory power of the analysis. Based on similarity coefficients 85% of strains had more than 65% homology. This data show that M. tuberculosis strains obtained in Mozambique, Africa can be analyzed for epidemiological purposes with the use of this genotyping technique. However, a considerable number of strains exhibited a low number of IS6110 copies, and a second genetic marker as spoligotyping has to be used

ASSUNTO(S)

rflp hiv (virus) polimorfismo polymorphism mycobacterium tuberculosis hiv (virus) aids (diseases) mycobacterium tuberculosis - moçambique aids (doença) - moçambique is6110 is6110 rflp

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