Fluorescence Enhancement of Laccase Induced by Reduction of Cu(II) Sites

AUTOR(ES)
RESUMO

The intrinsic fluorescence of laccase (p-diphenol:O2 oxidoreductase, EC 1.10.3.2), emitted by its tyrosinyl and tryptophanyl residues, underwent significant enhancement upon reduction of the enzyme redox sites. The increase in quantum yield reached its maximum after the addition of approximately four reduction equivalents and depended on the wavelength of excitation:

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