Fluctuation x-ray scattering from biological particles in frozen solution by using synchrotron radiation.

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RESUMO

Determination of the structure of biological particles, randomly oriented in solution, from spatial correlation analysis of fluctuations in x-ray scattering has recently been proposed. The feasibility of scattering fluctuation measurements was evaluated by using an x-ray synchrotron radiation camera to obtain the spatial correlation for a solution of tobacco mosaic virus along a line. The experimental system, analysis of data, and requirements for the determination of structures in solution are discussed using this example.

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