Fixation of dinitrogen derived from denitrification of nitrate in a photosynthetic bacterium, Rhodopseudomonas sphaeroides forma sp. denitrificans.
AUTOR(ES)
Dunstan, R H
RESUMO
Studies with 15N demonstrated that the phototrophic bacterium Rhodopseudomonas sphaeroides forma sp. denitrificans strain IL106 cannot assimilate NO-3 but rather denitrifies it to N2. This strain also fixed N2 into cell protein, although nitrogenase activity was partially inhibited in the presence of NO-3. Strain IL106 did not assimilate NO-3, but growing cultures and washed cell suspensions incorporated the tracer from 15NO-3 via denitrification to 15N2 and then via nitrogenase into cell nitrogen. This incorporation was inhibited in cells supplied with NH4+ or in the absence of light, thus confirming the participation of nitrogenase in the assimilation of nitrogen from nitrate. This represents a novel type of N2 recycling in a photodiazotrophic denitrifying bacterium.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=220086Documentos Relacionados
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