Exprimental infection of dogs (Canis familiaris) with sporulated oocysts of Neospora caninum. / Infecção experimental de cães (Canis familiaris) com oocistos esporulados de Neospora caninum.

AUTOR(ES)

Luciana Ahlf Bandini

DATA DE PUBLICAÇÃO

2009

RESUMO

The objective of this study was to evaluate the acquisition of infection in dogs experimentally infected with sporulated oocysts. The oocysts used as inoculum were obtained by bioassay in dogs using brain of buffaloes positive for anti-N. caninum antibodies. The oocysts were confirmed to be N. caninum by molecular methods and by bioassay in gerbils. Sporulated oocysts with a maximum of 90 days were used in the infection of four dogs. The dogs were eight weeks old and were negative for anti-N. caninum and Toxoplasma gondii antibodies. Dogs 1 and 4 received an inoculum of 10,000 sporulated oocysts each, dog 2 an inoculum with 5,000 sporulated oocysts and dog 3 received 1,000 sporulated oocysts of N. caninum. The total feces eliminated by the dogs were collected and examined daily for a period of 30 days. No oocysts were found in the feces of these animals. For six months the dogs were monitored to observe a possible seroconversion and when this occurred the animals were eliminated from the experiment.

ASSUNTO(S)

experimental animal infection neospora caninum dogs infecção experimental animal cães neospora caninum oócitos oocytes

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