Expression of homeobox genes in oral squamous cell carcinoma cell lines, stimulated with EGF and TGF-beta / Expressão de genes homeobox em células de carcinoma epidermóide de boca estimuladas com EGF e TGF-beta

AUTOR(ES)

Marcia Sampaio Campos

DATA DE PUBLICAÇÃO

2008

RESUMO

Homeobox genes, vital to many aspects related with cellular growth and differentiation, had been described as deregulated in some cancers. Their role in carcinogenesis, mainly oral squamous cell carcinomas, remains unclear and poorly characterized. Thus, this study had the purpose to evaluate, in cell cultures, the expression profile of six homeobox genes (ASH2L, HOXA7, HHEX, PKNOX1, PITX1, TGIF) selected among genes previously identified in the Head and Neck Cancer Genoma Project (2001), under stimulation with EGF and TGF-beta1. Oral squamous cell carcinoma cell lines from primary tumour (HN6) and from methastasis (HN31), and a non-tumoral cell line (HaCat) were cultured under standard procedures. CDNAs were obtained by RT-PCR and the transcripts were amplified and quantified by real-time PCR. Data were normalized by HPRT gene and the relative quantification was made by the delta Ct method. According to the results, it was possible to observe that EGF produced a variable modulation of the analyzed genes, in all cell lines. Generally, TGF-beta1 was able to significantly increase (ANOVA, p<0,05) the expression of the transcripts of 5 homeobox genes (HOXA7, HHEX, PKNOX1, PITX1, TGIF). Transcripts of PITX1 and TGIF genes were particularly more expressed in the tumoral cell lines (HN6 e HN31), when compared to the non-tumoral cell line, when treated with TGF-beta1. It is suggested that the studied homeobox genes play different roles in oral squamous cell carcinoma and that, especially the PITX1 and TGIF act as oncogenes, inhibitting the TGF-dependent anti-proliferative response, leading to tumour progression.

ASSUNTO(S)

egf tgf-beta genes homeobox linhagens celulares qpcr oral squamous cell carcinoma tgf-beta carcinoma epidermóide de boca egf cell lines homeobox genes pcr quantitativo

Documentos Relacionados

• Perfil da expressão de genes homeobox em linhagens celulares de carcinoma epidermóide de boca estimuladas pelo ácido retinóico
• Homeobox genes transcripts expression in oral squamous cell carcinoma: microarray analysis, qRT-PCR validation and association with clinical criteria of aggressiveness
• Efeito apoptótico do Celecoxib em linhagens celulares derivadas de carcinoma epidermóide de boca
• A role of the latent TGF-beta 1-binding protein in the assembly and secretion of TGF-beta 1.
• COX-2 expression analysis and signalling pathway mechanisms in human oral squamous cell carcinoma cell lines