Expression of Helicobacter pylori Vacuolating Toxin in Escherichia coli
AUTOR(ES)
McClain, Mark S.
FONTE
American Society for Microbiology
RESUMO
VacA is a secreted toxin that plays a role in Helicobacter pylori colonization of the stomach and that contributes to the pathogenesis of peptic ulcer disease. Studies of VacA structure and function have been hindered by the lack of an efficient system for expression and genetic manipulation of this toxin. In this study, we developed methodology for expression of a functionally active VacA toxin in Escherichia coli. We then used a high-throughput screen to analyze a library of mutant toxins with pentapeptide insertions and identified six mutants that lacked the capacity to induce vacuolation of HeLa cells. The capacity to analyze VacA in this heterologous-expression system should greatly facilitate efforts to elucidate the structure and function of this toxin.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=152094Documentos Relacionados
- Carboxy-Terminal Proteolytic Processing of Helicobacter pylori Vacuolating Toxin
- Clustering and Redistribution of Late Endocytic Compartments in Response to Helicobacter pylori Vacuolating Toxin
- Pathological significance and molecular characterization of the vacuolating toxin gene of Helicobacter pylori.
- Membrane channel structure of Helicobacter pylori vacuolating toxin: Role of multiple GXXXG motifs in cylindrical channels
- Selective increase of the permeability of polarized epithelial cell monolayers by Helicobacter pylori vacuolating toxin.