Expressão espaço-temporal de marcadores de poliferação e morte celular na sinfise pubica de camundongo durante a prenhez, parto e pos-parto

AUTOR(ES)
DATA DE PUBLICAÇÃO

2005

RESUMO

Marked changes in mice pubic symphysis occurs by the end of pregnancy. Recently, we demonstrated that these changes in the pubic symphysis during the period under study are directly associated with extracellular matrix components remodeling mainly collagen, elastic fibers system, proteoglycans and glycosaminoglycans. Tissue remodeling involves a dynamic balance between cell proliferation and programmed cell death as well as changes in the extracellular matrix components. Therefore, it is important to consider these both cellular behaviors when investigating the mechanism that regulates the interpubic tissue remodeling; growth during Iate pregnancy and partum ensuring involution during the postpartum period. Proliferating and programmed death cells were identified by immunohistochemistry, PCNA and TUNEL detection, respectively, and the rates at which these processes occurred were determined by morphometric analysis. Our results demonstrated that cellular proliferation was intense during the period of ligament formation, from 015 to 018, thereafter abruptly declining on 019. From parturition (019) onwards, an ever increasing decline in the cellular proliferation levels could be observed. The quantitative analyses of cellular death, showed opposite results when compared to cellular proliferation. For example, on 019, has initiated the decrease on the proliferative index. Likewise, on this day that occur one of the highest levei of cellular death. When we observed simultaneously proliferation and cellular death, during the early pregnancy the cycle of cellular renovation is clearly proliferative, producing hyperplasia for in the later pregnancy the cycle be directing by programmed cellular death. Interestingly, although the high levels of the cellular death during postpartum involution could be show by the TUNEL positive cells, we were unable to observed picnotic nucleus. We would expect to find picnotic nucleus with reasonable frequency in this situation, where programmed cellular death occurs in a great extension. The process of autophagy, a variation of classical apoptotic cellular death was discussed as an alternative mechanism of the cell elimination in the postpartum Iigament involution.

ASSUNTO(S)

prenhez pregnancy proliferação celular cell proliferation sinfise pubiana morte celular pubic symphysis

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