Evidence that infectious pancreatic necrosis virus has a genome-linked protein.

AUTOR(ES)

Persson, R H

RESUMO

The double-stranded RNA segments of infectious pancreatic necrosis virus were extracted from virions by a method which avoids proteinase. In contrast to proteinase-treated RNA, such segments (i) exhibited a lower electrophoretic mobility in sodium dodecyl sulfate-polyacrylamide gels and agarose gels, (ii) had a slightly lower buoyant density, and (iii) demonstrated a marked tendency toward aggregation as observed by electron microscopy. A small amount of protein tightly bound to the RNA could account for the above properties, and a 110,000-dalton protein was liberated from purified virion RNA by sequential digestion with RNase III and RNase A. The amount of radioactivity associated with RNA from virions labeled in vivo with [35S]methionine suggested that an average of 1.4 molecules was bound per RNA segment. Interactions between RNA segments seen in electron micrographs appeared to occur only among the ends of the segments, suggesting these were the exclusive sites of protein attachment.

Documentos Relacionados

• Three-dimensional model of the potyviral genome-linked protein.
• Heterogeneity of the genome-linked protein of foot-and-mouth disease virus.
• Mutational analysis of the genome-linked protein VPg of poliovirus.
• Membrane-dependent uridylylation of the genome-linked protein VPg of poliovirus.
• A Cysteine-Rich Plant Protein Potentiates Potyvirus Movement through an Interaction with the Virus Genome-Linked Protein VPg