Evaluation of the Commercial Kit SIRE Nitratase for detecting resistant Mycobacterium tuberculosis in Brazil
AUTOR(ES)
Miranda, Silvana Spindola de, Almeida, Isabela Neves de, Lopes, Maria Luiza, Figueiredo, Jamilly dos Reis de, Figueredo, Lida Jouca de Assis, Kritski, Afrânio Lineu, Carvalho, Wânia da Silva, Mansur, Maria de Fátima Filardi Oliveira
FONTE
Rev. Soc. Bras. Med. Trop.
DATA DE PUBLICAÇÃO
2017-08
RESUMO
Abstract INTRODUCTION: This study aimed to evaluate a new commercial kit, Kit SIRE Nitratase-PlastLabor, for testing the drug susceptibility of clinical Mycobacterium tuberculosis isolates. METHODS: The accuracy of the Kit SIRE Nitratase was evaluated by examining the susceptibility (streptomycin, isoniazid, rifampicin, and ethambutol) of 40 M. tuberculosis isolates, using the proportion method with Lowenstein-Jensen medium or the BACTEC MGIT 960 system. RESULTS: The detection accuracy for streptomycin, isoniazid, rifampicin, and ethambutol was 95%, 97.5%, 100%, and 80%, respectively. CONCLUSIONS: The exceptional accuracy demonstrated by Kit SIRE Nitratase for isoniazid and rifampicin makes the kit an attractive option for screening M. tuberculosis strain resistance.
Documentos Relacionados
- Evaluation of a commercial ligase chain reaction kit (Abbott LCx) for direct detection of Mycobacterium tuberculosis in pulmonary and extrapulmonary specimens.
- Use of a commercial PCR kit for detecting Chlamydia trachomatis.
- Evaluation of a line probe assay kit for characterization of rpoB mutations in rifampin-resistant Mycobacterium tuberculosis isolates from New York City.
- Evaluation of a commercial radioisotopic kit for folate assays.
- Evaluation of three commercial detection systems for Mycobacterium tuberculosis where clinical diagnosis is difficult.