Estudo epidemiólogico-molecular e de fatores de virulência de Staphylococcus aureus associados à mastite bovina em propriedades de exploração leiteira dos Estados de São Paulo e Pernambuco. / Molecular epidemiology and virulence factors of Staphylococcus aureus associated to bovine mastitis in dairy herds from São Paulo and Pernambuco state.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

A total of 107 S. aureus isolated from bovine milk, udder skin, milkers and milking machine, from São Paulo and Pernambuco herds was typed by molecular techniques. PCR-RFLP coa gene and PCR spa gene distinguished six amplicons. All strains amplified coa, spa, icaA genes and, 69% produced in vitro glucose-induced biofilm. PFGE identified 31 pulsotypes, 12 lineages. One of the lineages was predominantly isolated (P<0.0001) and widely disseminated. A same pulsotype was isolated from clinical and, subclinical mastitis as well as from carriers. There was genetic heterogeneity among isolates from the herds. Strains from human and animal origin were genetically different. Few isolates from milk, milking machine and udder skin showed similar pulsotype. An extramammary strain, 77% of the milk isolates and, 99% of the S. aureus isolated from carriers produced biofilm. It was not detected any correlation between SCC and biofilm production. The successive isolation during more than 16 days of a same pulsotype from the asymptomatic glands characterized the carrier status.

ASSUNTO(S)

staphylococcus aureus biofilmes carrier status mastite bovina biofilms pfge e pcr (reação em cadeia por polímerase) portador de doença animal epidemiologia molecular pfge and pcr (polymerase chain reaction) microbiology microbiologia molecular epidemiology bovine mastitis staphylococcus aureus

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