Estudo do modelo de quimeras de medula óssea (WT/IFNgR-KO) para examinar o papel do IFN-g sobre as células não leucocitárias na infecção pelo Trypanosoma cruzi. / Analysis of the model of bone marrow chimeras (WT/IFNg-R-KO) to examine the role of IFNK-g upon non leukocytes in Trypanosoma cruzi infection.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

Although we know the role played by leukocytes in Trypanosoma cruzi control, we ignore the contribution of structural cells (myocytes, hepatocytes, etc). These cells could signal the presence of the parasite and/or mediate an effector activity which could increase in response to cytokines, as IFN-g. To evaluate if non-leukocytes respond to IFNg, contributing to T. cruzi destruction, we analysed the infection in WT/IFNgR-KO (WT/KO) chimaeras, generated in IFNgR-KO mice reconstituted with bone marrow of wild-type mice (WT) so that most leukocytes are IFNgR+ but structural cells are deficient. WT/KO chimaeras and control WT/WT chimaeras were infected by T. cruzi and the parasite load and inflammation analyzed in various organs. Systemic and tissue parasite loads were higher in WT/KO chimeras than in WT/WT chimeras, but lower than in control IFNgR-KO mice. In WT/KO chimaeras the increased parasite load at the heart and skeletal muscle was not followed by an increase of inflammatory infiltrates. Our results suggest that structural cells contribute to T. cruzi control.

ASSUNTO(S)

non-leucocyte cells trypanosoma cruzi bone-marrow chimeras ifn-g trypanosoma cruzi immune response células não leucócitárias ifn-g resposta imune quimeras de medula óssea

ACESSO AO ARTIGO

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