Estudo da migração de neutrofilos in vitro em coelhos hepatopatas

AUTOR(ES)
DATA DE PUBLICAÇÃO

2001

RESUMO

The aim of this study was to investigate neutrophil chemotaxis during the induction of liver cirrhosis in rabbits. The study had three expérimentai arms: Group A (n=16) served as controls and received only normal rabbit chow. All animals were sacrificed at 16 weeks. Group B (n=19) was sacrificed 48 h after the chemotaxis assay, which was performed 24 h after CCL4 administration, at weeks 2, 4, 6 and 8. Group C (n=8) was followed by 16 weeks. The chemotaxis assay was performed 1 week after CCU administration. All animals were sacrificed at 16 weeks. The rabbits were treated with intragastric administration of CCU once weekly starting 14 days after addition of Phénobarbital to the drinking water (50mg/day). The CCU dose was adjusted weekly in order to obtain serun ALT and AST levels between 400 and 800 IU/I. Our results showed that increased ALT and AST levels were found in all of CCU-treated rabbits compared to the controls. In liver from animals sacrificed in 2, 4, 6 and 8 weeks of CCU treatment, hepatitis and fibrosis were already found at week two, and liver cirrhosis at week 6 and 8. Sixteen-week CCU treatment resulted in liver cirrhosis in 7 animals (87.5%). All animals with liver cirrhosis had an inflammatory infiltrate with neutrophils. Treatment with CCU did not interfered with total leukocyte count in blood from animals sacrificed in different periods, but a significant increase in polymorphonuclear cells between weeks 2 to 8 and a significant reduction in mononuclear leukocytes at week 4 were observed when compared with control animals. Neutrophil chemotaxis was also significantly increased from week 2 through week 8 compared with the control group. Animals that received CCU for 16 weeks present a significantly increased total leukocyte and polymorphonuclear number at week 10 when compared to controls. The mononuclear cell number was unchanged during the study period. A significant increase in neutrophil chemotaxis was observed from week 2 trough week 6. The presence of neutrophils in the liver of all animals with cirrhosis associated with an increase in polymorphonuclear cell chemotaxis during CCL4 administration in our experiment supports the view that this cell line has an important role in the development of toxic liver damage

ASSUNTO(S)

migração neutrofilos cirrose hepatica

ACESSO AO ARTIGO

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