Estimation of Somatomedin-C Levels in Normals and Patients with Pituitary Disease by Radioimmunoassay
AUTOR(ES)
Furlanetto, Richard W.
RESUMO
The development of a radioimmunoassay for somatomedin-C has for the first time made it possible to discriminate between serum concentrations of a single peptide or closely related group of peptides and the net somatomedin activity measured by less specific bioassay and radioreceptor techniques. Antibodies to human somatomedin-C were raised in rabbits using a somatomedin-C ovalbumin complex as the antigen. A variety of peptide hormones at concentrations up to 1 μM are not recognized by the antibody. Insulin at concentrations >0.1 μM cross reacts in a non-parallel fashion; purified somatomedin-A is only 3% as active as somatomedin-C; and radiolabeled cloned rat liver multiplication stimulating activity does not bind to the antibody. Immunoreactive somatomedin-C can also be quantitated in the sera of a variety of subhuman species.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=372409Documentos Relacionados
- Dietary components that regulate serum somatomedin-C concentrations in humans.
- Optimizing the expression in E. coli of a synthetic gene encoding somatomedin-C (IGF-I).
- Sequential addition of platelet factor and plasma to BALB/c 3T3 fibroblast cultures stimulates somatomedin-C binding early in cell cycle.
- The Micro Estimation of Serum Iron and Iron-binding Capacity in Normals and in Disease
- Radioimmunoassay of somatomedin B. Application to clinical and physiologic studies.